LOCUS       40924_21618        3249 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pGFPTA DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Ito Y, Suzuki M, Husimi Y.
  TITLE     A T-extended vector using a green fluorescent protein as an 
            indicator
  JOURNAL   Gene 245 (1), 59-63 (2000)
  PUBMED    10713445
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Ito Y, Suzuki M, Husimi Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2000) Yuzuru Husimi, Saitama University, 
            Deptartment of Functional Materials Science; 255 Shimo-ookubo, 
            Urawa, Saitama 338-8750, Japan 
            (E-mail:ito@evolve.fms.saitama-u.ac.jp, Tel:+81-48-858-3531, 
            Fax:+81-48-858-3531)
REFERENCE   3  (bases 1 to 3249)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3249)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "2000"; volume: "245"; issue: "1"; pages: "59-63"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-FEB-2000) Yuzuru Husimi, Saitama University, Deptartment of 
            Functional Materials Science"; volume: " 255 Shimo-ookubo, Urawa, 
            Saitama 338-8750, Japan (E-mail:ito@evolve.fms.saitama-u.ac.jp, 
            Tel:+81-48-858-3531, Fax"; pages: "+81-48-858-3531"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3249
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    52..73
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        88..118
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    126..142
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     150..166
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             234..947
                     /codon_start=1
                     /label=GFPuv
                     /note="GFP variant optimized for excitation by UV light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALLKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     misc_feature    952..957
                     /label=EcoRI/PvuII restriction site
                     /note="EcoRI/PvuII restriction site"
     promoter        1058..1162
                     /label=AmpR promoter
     CDS             1163..2020
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2194..2782
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(2968..3108)
                     /label=bom
                     /note="basis of mobility region from pBR322"
ORIGIN
        1 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
       61 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
      121 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
      181 gcttgcatgc ctgcaggtcg actctagagg atccccgggt accggtagaa aaaatgagta
      241 aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta
      301 atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta
      361 cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta
      421 ctctgacgta tggtgttcaa tgcttttccc gttatccgga tcatatgaaa cggcatgact
      481 ttttcaagag tgccatgccc gaaggttatg tacaggaacg cacgatatct ttcaaagatg
      541 acgggaacta caagacgcgt gctgaagtca agtttgaagg tgataccctt gttaatcgta
      601 tcgagttaaa aggtattgat tttaaagaag atggaaacat tctcggacac aaactcgagt
      661 acaactataa ctcacacaat gtatacatca cggcagacaa acaaaagaat ggaatcaaag
      721 ctaacttcaa aattcgccac aacattgaag atggatccgt tcaactagca gaccattatc
      781 aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcga
      841 cacaatctgc ccttttgaaa gatcccaacg aaaagcgtga ccacatggtc cttcttgagt
      901 ttgtaactgc tgctgggatt acacatggca tggatgagct ctacaaataa tgaattcttg
      961 aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     1021 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     1081 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     1141 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     1201 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     1261 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     1321 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     1381 gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat
     1441 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     1501 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     1561 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     1621 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     1681 cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac
     1741 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     1801 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     1861 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     1921 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     1981 acagatcgct gagataggtg cgtcactgat taagcattgg taactgtcag accaagttta
     2041 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     2101 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     2161 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     2221 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     2281 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     2341 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     2401 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     2461 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     2521 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     2581 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     2641 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     2701 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     2761 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     2821 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     2881 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     2941 gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg
     3001 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     3061 aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg
     3121 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     3181 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     3241 gcgaggcag
//
